In Vitro Fertilization is an assisted reproductive technology (ART) used for infertility treatment and that involves collecting eggs from the woman and retrieving a male sperm sample to combine them in a laboratory.
To trigger ovulation relevant medications are administered and egg retrieval takes place 34-36 hours after this final injection. Follicle aspiration is performed under light anesthesia, a simple harmless procedure, which lasts only 15-30 minutes, and to which patients respond very well. Using a special needle, follicular fluids are retrieved from the ovary under ultrasound monitoring. The patient spends several hours in recovery under doctor’s supervision.After the embryologist identifies eggs in follicle fluids aspirated from the ovarian follicles, examines them for maturity, and adds sperm in amounts necessary for fertilization. This process is called standard IVF (In Vitro Fertilization). Successful egg fertilization is confirmed 18 hours after the procedure, followed by its development monitored by the embryologist in the course of 24-72 hours.

In cases of successful in vitro fertilization (IVF), culture of the embryo continues in the laboratory for a period of between 2 and 5 days, sometime even 6 or 7 days. On the 5th day the most advanced embryos become blastocysts. The superb quality of blastocyst allows for transferring only one embryo, thereby mitigating the risk of multiple pregnancy.